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il1 β  (R&D Systems)


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    Structured Review

    R&D Systems il1 β
    Il1 β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il1+%CE%B2/Recombinant+Human+IL-1+beta%2FIL-1F2+Protein/pmc13006238-42-12-15
    Average 96 stars, based on 418 article reviews
    il1 β - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Study on pro-inflammatory effect and mechanism of galectin-9 (LGALS9) in osteoarthritis: Exacerbating inflammatory response by activating JNK and ERK1/2 pathways.
    Article Snippet: Galectin-9 (LGALS9) plays an important role in the occurrence and development of many diseases, including immunity, infection, cancer, etc.. Studies have found that LGALS9 can phosphorylate ERK1/2 in the MAPK pathway.. However, there is currently no clear conclusion on the role of LGALS9 in OA, and it is worth further exploring the regulatory role and mechanism of LGALS9 in OA in this study.

    Sterility:

    Article Title: Study on pro-inflammatory effect and mechanism of galectin-9 (LGALS9) in osteoarthritis: Exacerbating inflammatory response by activating JNK and ERK1/2 pathways.
    Article Snippet: Galectin-9 (LGALS9) plays an important role in the occurrence and development of many diseases, including immunity, infection, cancer, etc.. Studies have found that LGALS9 can phosphorylate ERK1/2 in the MAPK pathway.. However, there is currently no clear conclusion on the role of LGALS9 in OA, and it is worth further exploring the regulatory role and mechanism of LGALS9 in OA in this study.

    Transferring:

    Article Title: Study on pro-inflammatory effect and mechanism of galectin-9 (LGALS9) in osteoarthritis: Exacerbating inflammatory response by activating JNK and ERK1/2 pathways.
    Article Snippet: Galectin-9 (LGALS9) plays an important role in the occurrence and development of many diseases, including immunity, infection, cancer, etc.. Studies have found that LGALS9 can phosphorylate ERK1/2 in the MAPK pathway.. However, there is currently no clear conclusion on the role of LGALS9 in OA, and it is worth further exploring the regulatory role and mechanism of LGALS9 in OA in this study.

    Protein Extraction:

    Article Title: Study on pro-inflammatory effect and mechanism of galectin-9 (LGALS9) in osteoarthritis: Exacerbating inflammatory response by activating JNK and ERK1/2 pathways.
    Article Snippet: Galectin-9 (LGALS9) plays an important role in the occurrence and development of many diseases, including immunity, infection, cancer, etc.. Studies have found that LGALS9 can phosphorylate ERK1/2 in the MAPK pathway.. However, there is currently no clear conclusion on the role of LGALS9 in OA, and it is worth further exploring the regulatory role and mechanism of LGALS9 in OA in this study.

    Western Blot:

    Article Title: Study on pro-inflammatory effect and mechanism of galectin-9 (LGALS9) in osteoarthritis: Exacerbating inflammatory response by activating JNK and ERK1/2 pathways.
    Article Snippet: Galectin-9 (LGALS9) plays an important role in the occurrence and development of many diseases, including immunity, infection, cancer, etc.. Studies have found that LGALS9 can phosphorylate ERK1/2 in the MAPK pathway.. However, there is currently no clear conclusion on the role of LGALS9 in OA, and it is worth further exploring the regulatory role and mechanism of LGALS9 in OA in this study.

    Article Title: Macrophage-Specific Lactate Dehydrogenase Expression Modulates Inflammatory Function In Vitro
    Article Snippet: Image Studio Lite (LI-COR Biosciences) software was used for densitometry measurements. .. Antibodies to LDHA (Proteintech, Rosemont, IL; cat. 19987-1-AP, 1:5,000), LDHB (Proteintech, Rosemont, IL; cat. 19988-1-AP, 1:5,000), iNOS (Proteintech, Rosemont, IL; cat. 18985-1-AP, 1:1,000), Arg1 (Proteintech, Rosemont, IL; cat. 16001-1-AP, 1:1,000), ß-actin (Sigma, St. Louis, MO; cat. A2228, 1:10,000), IL1-β (R&D Systems Minneapolis, MN; cat. AF-401-NA, 1:1,000), p65 (Cell Signaling, Danvers, MA; cat. 8242, 1:2,500), p-p65 (Santa Cruz, Dallas, TX; cat. sc-136548, 1:1,000) were used for immunoblotting. .. A phagocytosis assay was performed using the Vybrant Phagocytosis Assay Kit (Thermo Fisher Scientific, Waltham, MA; cat. V6694) following the manufacturer’s protocol.

    Purification:

    Article Title: CD40-directed scFv-TRAIL fusion proteins induce CD40-restricted tumor cell death and activate dendritic cells
    Article Snippet: .. Cells were then treated overnight with the various TRAIL fusion proteins, Killer-TRAIL, scFv:G28-TNC-GpL and gold standard, a mixture composed of TNF (purified protein, produced using HEK293 cells), IL1 β (R&D Systems), IL-6 (Miltenyi Biotec) and prostaglandin E2 (PGE2, Biomol, Hamburg, Germany), to evaluate the DC-stimulating activities of these reagents. ..

    Produced:

    Article Title: CD40-directed scFv-TRAIL fusion proteins induce CD40-restricted tumor cell death and activate dendritic cells
    Article Snippet: .. Cells were then treated overnight with the various TRAIL fusion proteins, Killer-TRAIL, scFv:G28-TNC-GpL and gold standard, a mixture composed of TNF (purified protein, produced using HEK293 cells), IL1 β (R&D Systems), IL-6 (Miltenyi Biotec) and prostaglandin E2 (PGE2, Biomol, Hamburg, Germany), to evaluate the DC-stimulating activities of these reagents. ..

    Transduction:

    Article Title: Syntaxin 4 protects islet β-cells from cytokine-induced senescence
    Article Snippet: .. Following transduction, islets were treated with a proinflammatory cytokine cocktail consisting of IL1-β (1 ng/mL, R&D System, cat #201-LB/CF, Minneapolis, MN), TNF-α (5 ng/mL, R&D System, cat #210-TA/CF), IFN-γ (40 ng/mL, R&D System, cat #285-IF/CF) for 48 h. .. MIN6 cells and human islets were harvested and lysed with RIPA buffer (Thermo Fisher Scientific) supplemented with 1x Halt Complete Protease and Phosphatase Inhibitor Cocktail, EDTA-free (Thermo Fisher Scientific).

    other:

    Article Title: Effects of Physical Exercise and Motor Activity on Depression and Anxiety in Post-Mastectomy Pain Syndrome
    Article Snippet: The following kits were used: IL-17 (R&D System; Manufactured and Distributed by: USA R&D Systems, Inc. 614 McKinley Place NE, Minneapolis, MN 55413, USA, Catalog #: D1700); IL1-β (R&D System Catalog #: DLB50); BDNF (R&D System Catalog #: DBD00); ACTH (Novus biologicals; Novus Biologicals USA 10730 E. Briarwood Avenue Centennial, CO 80112, USA; NBP2-66401); and cortisol (Novus biologicals NBP3-18003).



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    miR140@MVs inhibit the pro-inflammatory effect of LPS-induced BMDM in vitro . (A) The intracellular location of Cy3-miR140@FITC-MVs in BMDM cells stimulated with LPS (100 ng/mL) for 24 h (M1 macrophages). miR140 is labeled with Cy3 (Red) and MVs are stained by FITC-Annexin V (Green). The nuclei were stained by Hoechst 33342 (Blue). Scale bar: 5 μm. (B) Representative flow cytometric analysis of BMDM treated with different formulations. The (C) M1 (CD86 + CD206 – ) and (D) M2 (CD86 − CD206 + ) phenotypes were analyzed by Flowjo. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with M1 group. n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗∗ P < 0.0001. (E) Western blotting of TLR4 in LPS-induced BMDM treated with different formulations. (F) The relative expression of the protein band was analyzed by Image J and normalized to its respective baseline controls. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. ∗ P < 0.05. The relative mRNA levels of (G) TNFα , (H) IL6 , (I) IL1β , (J) IL10 , (K) TGFβ in LPS-induced BMDM receiving different formulations. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with the M1 group, n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (L) The TNF α level in LPS-induced BMDM supernatant was detected by ELISA assay. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. No significance, ns; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (F–I) Values are normalized to the M1 (baseline control) group.
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    miR140@MVs inhibit the pro-inflammatory effect of LPS-induced BMDM in vitro . (A) The intracellular location of Cy3-miR140@FITC-MVs in BMDM cells stimulated with LPS (100 ng/mL) for 24 h (M1 macrophages). miR140 is labeled with Cy3 (Red) and MVs are stained by FITC-Annexin V (Green). The nuclei were stained by Hoechst 33342 (Blue). Scale bar: 5 μm. (B) Representative flow cytometric analysis of BMDM treated with different formulations. The (C) M1 (CD86 + CD206 – ) and (D) M2 (CD86 − CD206 + ) phenotypes were analyzed by Flowjo. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with M1 group. n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗∗ P < 0.0001. (E) Western blotting of TLR4 in LPS-induced BMDM treated with different formulations. (F) The relative expression of the protein band was analyzed by Image J and normalized to its respective baseline controls. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. ∗ P < 0.05. The relative mRNA levels of (G) TNFα , (H) IL6 , (I) IL1β , (J) IL10 , (K) TGFβ in LPS-induced BMDM receiving different formulations. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with the M1 group, n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (L) The TNF α level in LPS-induced BMDM supernatant was detected by ELISA assay. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. No significance, ns; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (F–I) Values are normalized to the M1 (baseline control) group.
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    miR140@MVs inhibit the pro-inflammatory effect of LPS-induced BMDM in vitro . (A) The intracellular location of Cy3-miR140@FITC-MVs in BMDM cells stimulated with LPS (100 ng/mL) for 24 h (M1 macrophages). miR140 is labeled with Cy3 (Red) and MVs are stained by FITC-Annexin V (Green). The nuclei were stained by Hoechst 33342 (Blue). Scale bar: 5 μm. (B) Representative flow cytometric analysis of BMDM treated with different formulations. The (C) M1 (CD86 + CD206 – ) and (D) M2 (CD86 − CD206 + ) phenotypes were analyzed by Flowjo. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with M1 group. n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗∗ P < 0.0001. (E) Western blotting of TLR4 in LPS-induced BMDM treated with different formulations. (F) The relative expression of the protein band was analyzed by Image J and normalized to its respective baseline controls. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. ∗ P < 0.05. The relative mRNA levels of (G) TNFα , (H) IL6 , (I) IL1β , (J) IL10 , (K) TGFβ in LPS-induced BMDM receiving different formulations. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with the M1 group, n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (L) The TNF α level in LPS-induced BMDM supernatant was detected by ELISA assay. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. No significance, ns; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (F–I) Values are normalized to the M1 (baseline control) group.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Dual metabolic-inflammation modulation in MicroRNA@neutrophil-derived microvesicles achieve robust osteoarthritis therapy

    doi: 10.1016/j.apsb.2025.09.020

    Figure Lengend Snippet: miR140@MVs inhibit the pro-inflammatory effect of LPS-induced BMDM in vitro . (A) The intracellular location of Cy3-miR140@FITC-MVs in BMDM cells stimulated with LPS (100 ng/mL) for 24 h (M1 macrophages). miR140 is labeled with Cy3 (Red) and MVs are stained by FITC-Annexin V (Green). The nuclei were stained by Hoechst 33342 (Blue). Scale bar: 5 μm. (B) Representative flow cytometric analysis of BMDM treated with different formulations. The (C) M1 (CD86 + CD206 – ) and (D) M2 (CD86 − CD206 + ) phenotypes were analyzed by Flowjo. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with M1 group. n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗∗ P < 0.0001. (E) Western blotting of TLR4 in LPS-induced BMDM treated with different formulations. (F) The relative expression of the protein band was analyzed by Image J and normalized to its respective baseline controls. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. ∗ P < 0.05. The relative mRNA levels of (G) TNFα , (H) IL6 , (I) IL1β , (J) IL10 , (K) TGFβ in LPS-induced BMDM receiving different formulations. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, compared with the M1 group, n = 3. No significance, ns; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (L) The TNF α level in LPS-induced BMDM supernatant was detected by ELISA assay. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. No significance, ns; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. (F–I) Values are normalized to the M1 (baseline control) group.

    Article Snippet: Cytokine concentration in the supernatant was quantified by mouse TNF α or mouse IL1 β enzyme-linked immunosorbent assay (ELISA) kits (Elabscience, USA).

    Techniques: In Vitro, Labeling, Staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Control

    Single miR140@MVs for prolonged OA treatment. (A) Schematic illustration of the administration regimen. (B) The relative miR140 level in the whole-knee joint of DMM mice treated with different formulations for 28 days. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. The normal group served as the control. No significance, ns. (C) Typical images of imageological examinations, histological analysis, TUNEL staining and immunohistochemical analysis. Red arrows: synovial inflammation; Yellow arrows: cartilage defect; Blue arrows: bone marrow edema; Red circles: periarticular osteophytes. M: meniscus; S: synovial; C: cartilage. Scale bars in H&E and safranine O-fast green staining: 100 μm; in TUNEL staining and immunohistochemical analysis: 50 μm. (D) Heatmap of variables of histological scoring in each group. (E) OARSI grades of the mice joints in each group. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. The normal group served as the control. No significance, ns; ∗∗∗∗ P < 0.0001. (F–J) The relative mRNA levels of inflammatory factors in OA joints from mice after different treatments, including (F) TNFα , (G) IL6 , (H) IL1β , (I) IL10 and (J) TGFβ . The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. The normal group served as control. No significance, ns; ∗∗ P < 0.01; ∗∗∗∗ P < 0.0001.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Dual metabolic-inflammation modulation in MicroRNA@neutrophil-derived microvesicles achieve robust osteoarthritis therapy

    doi: 10.1016/j.apsb.2025.09.020

    Figure Lengend Snippet: Single miR140@MVs for prolonged OA treatment. (A) Schematic illustration of the administration regimen. (B) The relative miR140 level in the whole-knee joint of DMM mice treated with different formulations for 28 days. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. The normal group served as the control. No significance, ns. (C) Typical images of imageological examinations, histological analysis, TUNEL staining and immunohistochemical analysis. Red arrows: synovial inflammation; Yellow arrows: cartilage defect; Blue arrows: bone marrow edema; Red circles: periarticular osteophytes. M: meniscus; S: synovial; C: cartilage. Scale bars in H&E and safranine O-fast green staining: 100 μm; in TUNEL staining and immunohistochemical analysis: 50 μm. (D) Heatmap of variables of histological scoring in each group. (E) OARSI grades of the mice joints in each group. The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. The normal group served as the control. No significance, ns; ∗∗∗∗ P < 0.0001. (F–J) The relative mRNA levels of inflammatory factors in OA joints from mice after different treatments, including (F) TNFα , (G) IL6 , (H) IL1β , (I) IL10 and (J) TGFβ . The data are shown as mean ± SEM and analyzed by one-way ANOVA test with Tukey's correction, n = 3. The normal group served as control. No significance, ns; ∗∗ P < 0.01; ∗∗∗∗ P < 0.0001.

    Article Snippet: Cytokine concentration in the supernatant was quantified by mouse TNF α or mouse IL1 β enzyme-linked immunosorbent assay (ELISA) kits (Elabscience, USA).

    Techniques: Control, TUNEL Assay, Staining, Immunohistochemical staining